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Using protein fractions prepared from WT and AS11 mid-developing siliques containing embryos at mid-development (Stages 6-8 as designated in the "Methods" section) we were able to determine the relative changes of TAG assembly activity in the mutant line.
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Method names are as defined in the methods section, except for CONS,MED,AVG and HSO.
As mentioned in the Methods section, the sequence variants were designated in accordance with the Human Genome Variation Society recommendations (http://www.hgvs.org/mutnomen/).hgvs.org/mutnomen/
To provide a functional CiaRH system for csRNA9 expression, the gene, designated ccnI, was amplified from plasmid pST0 and cloned into the S. pneumoniae integration vector pMRT2-2 [ 42] as described in the Methods section.
As described in the Methods section (Sect.
PLGA scaffolds were seeded as described in the methods section.
We apply the proposed method to GAW16 simulated data sets as described in the Methods section.
The data was normalized using the print tip loess method as described in the methods section.
as described in the Methods section.
Genotype groups were generated as described in the Methods section.
EMSA runs were performed as described in the methods section.
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