The phrase "as described using" is correct and usable in written English.
It can be used when referring to a description that is provided in a specific manner or format.
Example: "The process will be implemented as described using the guidelines provided in the manual."
Alternatives: "as outlined with" or "as detailed through".
To examine the effect of cell passaging on the media preparation, CM was also collected as described using passage 18 HFFs.
The RFA electrode model, tetrahedral mesh, and FEM analysis of the Penne's bioheat equation were simulated as described using COMSOL (FEMLAB) 5.0 [COMSOL, Stockholm, Sweden].
Total starch of the cooked rice was determined as described using the Megazyme total starch kit (Megazyme, Wicklow, Ireland) (AACC Standard 76 13.01).
Feeding RNAi was then performed as described, using the N2 strain [31].
Afterwards immunohistochemistry was performed as described using a primary antibody anti-BrdU (Dakocytomation, Denmark, 1/200).
Correlative EM was performed as described using cells growing on marked coverslips [23], [24].
Total RNA was isolated from all colonic specimens obtained as described using TRI Reagent Molecular Research Center , Inc).
Other yeast two-hybrid assays were done as described using S. cerevisiae strain PJ69-4A and the pGBT9 and pGAD vectors [28].
Immunodetection of α-smooth muscle actin (αSMA) on liver sections was carried out as described using a monoclonal anti-αSMA antibody (#A2547) from Sigma (St . Louis MO) [22].
Immunolocalization was carried as described using phosphate buffered saline (pH 8.0) containing 0.1% Tween 20 and 5% bovine serum albumin as a blocking agent [17].
Mouse-specific primers were designed (Table S1) and real-time PCR was carried out in triplicate as described, using 18 s primers for internal controls [15].
