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The whole training process of the DBNs is conducted as described in the steps below.
In order to achieve different degrees of tissue positivity, P1 and P2 were initially diluted up to 50% or 6.25% with N1 and N2, respectively, and the resulting mixtures then used for precipitating PrPSc as described in the steps above.
Instead, apply gentle heat (as described in the steps above).
This is a very simple task as described in the steps above.
Power on your computer as described in the steps describing how to start in Normal Mode.
Then ensure that the improper fraction has the same denominator as the other fractions you're dealing with, and add or subtract as described in the Steps.
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First-strand cDNA synthesis was performed in a thermal cycler (Life Technologies) with conditions as described in the step-wise protocol.
A StepOnePlus™ Real-Time PCR System (Life Technologies) was used for thermal cycling as described in the step-wise protocol.
Cells were suspended at a concentration of 50 500 cells/mL in increments of 50 cells and seeded onto Terasaki plates as described in the step-wise protocol.
Samples were harvested from individual wells containing single cells, kept at -80°C until further use (less than one week) and subsequently processed for RNA extraction and purification as described in the step-wise protocol.
After your last raka'at, complete the prayer after the second prostration by reciting the Tasyahhud, Salawat, Durud, and Salaam, as described in the step above.
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