CoP considers each tuple as a player and tuples form coalitions as described in the procedure.
cGMP content was assayed as described in the procedure of an ELISA kit obtained from Cayman Chemical & Co. cGMP level was normalized to tissue protein content in mg/ml.
Nitric acid (2 M volumetric standard, Riedel-de Haën) was used for electrode cleaning, as described in the " Procedure" section.
The mercury plating solution was prepared in nitric acid from a 1000 ppm mercury II) chloride (98+%, Aldrich) stock solution, as described in the " Procedure" section.
Further, the time course of STI-1-Flag expression after intracerebral injection of LV-STI-1-Flag in the ipsilateral ischemic brain was examined by Western blot using Flag (1 400; Sigma) antibody as described in the procedure above.
Bismuth plating solutions were prepared with bismuth III) nitrate pentahydrate (99.999%, Aldrich,) either in acetate buffer or with lithium bromide (99+%, Aldrich) in hydrochloric acid (1 M volumetric standard, Riedel-de Haën, Gillingham, UK), as described in the " Procedure" section.
As described in the Procedure subsection (Methods section), two genes that defined two sample groups which had at least one gene significantly different between the two sample groups were used to hierarchically cluster the training dataset samples.
The system was subsequently generated in about 24 h, and analyzed as described in the general procedure.
FAM105B/OUTLIN was identified bioinformatically with generalized profile analysis, as described in the Extended Experimental Procedures.
Plates were scanned with the Operetta High Content Imaging System (PerkinElmer) and analyzed as described in the Supplemental Experimental Procedures.
