Sentence examples for as described in the instructions from inspiring English sources
After cultivation, cell lysate of harvested E. coli cells was prepared using xTracter Buffer (Takara-Bio, Shiga, Japan) and recombinant wild-type BGLII (rBGLIIwt) was purified by TALON® Metal Affinity Resins (Takara-Bio, Shiga, Japan) as described in the instructions.
In vitro TNT T7 Quick Coupled Transcription/Translation System was conducted to synthesize the [35S]-radiolabeled DSS1-myc fusion protein as described in the instructions (Promega).
An internal standard, "alien RNA", was spiked into each sample to control for differences in cDNA conversion efficiency as described in the instructions for the Alien® QRT-PCR Inhibitor Alert kit (Stratagene, LaJolla, CA).
Further handling of sausages is as described in the instructions for fresh sausages for grilling.
The same PCR cycling was used as described in the instructions for use of the Epi proLung BL real-time PCR kit.
Nuclear extracts were prepared from Hep-2 cells using a nuclear extract kit (Active Motif, USA) as described in the instructions.
The quality control of the product was performed as described in the instruction leaflet.
Liver cytosolic GST activity were measured spectrophotometrically using CDNB as substrate as described in the instruction of the kit buying from (Biovision, USA).
PK2 mRNA-expressing cells were revealed with the TSA Plus Fluorescence Kit (Perkin Elmer, Waltham, MA) as described in the instruction manual.
Standard positioning was used as described in the instruction manual in all measurements and skin-to- skin contact was avoided.
The TruCulture® blood collection and whole-blood culture device (Myriad RBM, Inc., Austin, USA) was operated as described in the instruction manual.
