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The procedure was done exactly as described in the experiment above until trypsinization, then cells were stained with 2′7′-dichlorofluorescin diacetate (DCF-DA) (Sigma) (5 μg/ml) in PBS with Ca2+/Mg2+ for 30 min at 37 °C.
In case C2, No ATC alerts are generated as described in the experiment set-up.
Tissue and synovial cultures, and histopathological study of the synovia were performed as described in the experiment of arthritogenic ability of M. fermentans.
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As described in the Experiments section below, ChER utilises a comprehensive set of character and word n-grams as well as orthographic features, which were then augmented with ones which capture chemical knowledge, e.g., number of chemical basic segments, dictionary and chemical symbol matches.
Cells were plated, treated, and fixed as described in the experiments for TUNEL assay.
After the incubation, the samples were dried and digested, the peptide pools were labeled by d0- or d4-propylation, the samples were mixed in a 2 1 ratio and analyzed as described in the PKA experiment.
Nu/nu mice were inoculated with GBM43 tumour and treated exactly as described in the first experiment, with the exception of the choice of time points for evaluation of tumour MGMT activity.
This experiment was the same as described in the screening experiment, except we chose lower concentrations to determine the IC50 of NSC-124854.
For face identification we use the proposed system that combines global and local features for recognition which performed best in experiment 2. All parameters were set as described in the previous experiment.
Virus titration was performed as described in the chicken experiment.
The fMRI data were pre-processed and statistically analyzed as described in the original experiment.
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