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Then the probabilities were estimated as described in Step 2. Figure 1 shows the periods and age groups where breast cancer mortality data was available (in black) or estimated (in gray).
Up until this point, life had probably relied on RNA for most jobs (as described in Step 2 above).
Prepare glycerol stocks as described in Step 7. Glycerol stocks can usually be stored for several years at −80 °C.
The cover glass can be glued over the visible zone of the exposed lobe, as described in Step 19, after the incision in the muscle layer.
This can be performed as described in step (ii) leading to novel 'grand-child' variables.
The evolution of F F area in our model runs used data from country GHG inventories, as described in step 3 above.
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However, the number of sporozoites per female can be known only after counting as described in Steps 8 and 9. Once dissected, maintain salivary glands on ice.
For pre-embedding cytochemical detection proceed as described in steps 5 and 6 of Post-Embedding Immunocytochemical Detection).
Heat-shock and plate the cells on 96 separate LB plates containing kanamycin (50 μg ml−1), as described in Steps 4 6.
Identify mosquitoes that display GFP fluorescence in the salivary glands, and dissect these as described in Steps 5 10 (further details of the dissection can be found in ref. 20).
4. Inner Work: Always do your inner work as described in steps 1-3 abeforeefore deciding how to respond to the outer situation.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.
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CEO of Professional Science Editing for Scientists @ prosciediting.com