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Eight 2,3,7,8-TCDD 2,3,7,8-TCDDns (0.3–300 pM/well), dissolved in DMSO and proconcentrationsribed for sample extracts, were tested.
The analyses of samples D1 and D2, which contained the soluble and insoluble parts of the biological material, respectively, were performed according to the same procedure as described for sample C2.
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Vertebrate tissue and arthropod suspensions were processed and tested for filovirus nucleic acids by reverse transcription PCR (RT-PCR) and nested PCR by using filovirus-specific large (L) protein gene primers and nested MARV-specific viral protein 35 (Vprimersimers as described for samples from human patients during the outbreak (1 ).
Samples for protein expression were collected as described for RNA samples and transported to the laboratory in 0.9% NaCl at 4°C.
RNA-Seq data was collected as described for the human sample.
RNA was bound to silica-based columns and digested with DNase I as described for fresh-frozen tissue samples.
Phytophthora-like colonies were examined as described for those obtained from soil samples.
Primary analyses were conducted at an α level of P<0.0125 as described for the sample size calculation.
We classified complexes of all compounds from the ToxCast database with the five crystal structures using the GoldScore and hybrid score as described for the sample data set.
TSP were obtained as described: for each sample, 100 mg of leaf tissue was ground thoroughly in liquid nitrogen and the powder was added with 500 μl of PBS-buffer (PBS) containing protease inhibitors (Complete EDTA Free, ROCHE).
Groundwater was sampled and processed as described for hyporheic samples.
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