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However, as described below in section IX, paragraph b.3, the imposition of civil penalties will also take into account the gravity, circumstances, and extent of the violation or violations and, with respect to the violator, any history of prior similar violations and the degree of culpability and knowledge.
We then performed domain analysis on all hits as described below in section II.
Of these locations, three (1.5°, 3°, and 6°) were selected for analysis as described below in Section 2.3.
We estimate the P-value of score t using the empirical null score distribution we construct as described below in Section 2.4.
The objective function J is defined as follows: Here, λ i is a weight parameter for each feature f i, trained as described below in Section 2.4.3.
Consistent with these changes in the bioavailability of metal ions and as described below in section 4, FeSOD is the most ancient of the SODs and probably first appeared when Fe was relatively abundant.
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Screening of the control population for the sequence variants observed in patients was conducted by sequencing as described below in the section entitled PCR and DNA sequencing or, as indicated, by RFLP or TaqMan® analysis as described below in the sections entitled RFLP analysis and TaqMan® analysis.
The coding and near coding regions of all exons in all isoforms of SLC26A5 were sequenced in the patient population as described below in the section entitled PCR and DNA sequencing except that analysis of the IVS2-2A>G variant was conducted as reported previously [16].
The variants in conserved regions that had MAF information in dbSNP were genotyped, as described below in the section entitled "Genotyping of IDE variants", in at least the Mayo JS series to determine if they met the MAF cut-off of ≥0.01.
All groups of patients will receive treatment as usual improved by their GPs, as described below in this section.
Northern blot analysis were performed as described below in this section, using the full length cDNA sequence of PpHsp16.4 radiolabeled as a probe.
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