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When implanted in vivo, these scaffolds were able to retain their structural integrity over 1 month of implantation as demonstrated by serial ultrasonography.
Aldehyde dehydrogenase 1 gene expression and enzymatic activity are elevated in CoCSC and using an in vitro culture system that maintains CoCSC as demonstrated by serial transplants and lentiviral marking of single cell-derived clones, we further show that ALDH1 enzymatic activity is a major mediator of resistance to cyclophosphamide: a classical chemotherapeutic agent.
Trauma patients who do not receive prophylaxis, however, have DVT rates of 60%, as demonstrated by serial impedance plethys-mography and venography [ 11].
However, the addition of FCS at different concentrations slowed (10%) or entirely inhibited (25%) aggregation as demonstrated by serial measurement of hydrodynamic diameter over the course of 24 h.
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By definition, tumor-initiating cells are enumerated retrospectively, therefore altered CSC frequencies post-therapy are best demonstrated by serial transplantation.
This stem cell potential, including self-renewal and lineage capacity, was demonstrated by serial transplantation experiments in animal models.
Biotin signal was demonstrated by serial application of primary streptavidin-peroxidase, biotinyl-tyramide and secondary streptavidin-peroxidase [GenPoint Catalyzed Signal Amplification System] (DAKO).
However, graft functionality was further demonstrated by serial transplantation with retrieved grafts able to attenuate blood glucose levels in secondary recipient mice (Supplementary Figure S6C).
For serum-free cultures, the human counterparts of these same epitopes were presumably being detected; in such cases, it is also obvious that the initial seeding proteins would be diluted with each passage, until they would fade below detectable levels, as demonstrated by our own serial passage experiments.
The cells infiltrating the matrigel, removed after one week, expressed the myogenic marker MyoD along with the endothelial markers either CD34, VE-cadherin or Flk-1 [7], as demonstrated by double labelling experiments (CD34/MyoD, VE-cadherin/MyoD, Flk-1/MyoD) on serial sections (Fig. 4C E).
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CEO of Professional Science Editing for Scientists @ prosciediting.com