Of these men, one-hundred with ≥20×10/ml sperm concentration, ≥40×106 total sperm count, ≥2 ml semen volume, ≥50% of a+b or ≥25% a motility, high percentage of normal forms (≥10%) were selected as control population with normal spermatogenesis according to WHO criteria [17].
As control population we used 190 unrelated healthy relatives of patients recruited at Department of Clinical Genetics, Karolinska Hospital.
We analysed, as control population, 20 melanomas from our series, selected as they had never showed molecular MCAM/MUC18 expression and screened in line with matching for sex, age, ethnic background, primary tumour site and AJCC stages, as closely as possible.
Although the use of children with idiopathic nephrotic syndrome in remission as control population may be problematic when compared with "normal" children, there are ethical issues concerning studies with radiolabelled compounds in normal healthy children.
Control of territory, so vital in the heady early days, is no longer as important to Baghdadi and his senior leaders as controlling populations beyond the caliphate's fast-shrinking boundaries.
Attempts to manage the transition – such as controlling population movement while the North developed – could quickly be overtaken by events.
Of the 15 populations, six were selected as 'clean' control populations and the other nine were classified as originating from mining-impacted sites (Table S1).
So long as they control populations, it will be earning enough money to subsist and continue operations.
Low-risk orthopedic outpatient attendees (United Kingdom) and HIV-negative and HCV-negative nonremunerated blood donors (France) were used as negative control populations.
As a negative control, populations were left unstimulated and subsequently stained using the same protocol as described above.
