This provides clear evidence that a difference in the surfactant assembly on the sides as compared with the ends of the nanowires drives the directed assembly in this system.
We also discarded alignments to certain regions of the human genome for which the sequence is underrepresented in the sequence assembly as compared with the actual genome.
Star-shaped polymers, one kind of dendritic polymers with well-defined architecture and multiple polymer chains emanating from the central core, have similar topological structures to polymeric micelles and can form more stable nanoscale assemblies in selective solvents, as compared with the corresponding linear block analogues.
Combining different libraries typically did not improve the assemblies compared with the assembly of just library 24 (genome assembly quality has previously been shown to saturate and even worsen as sequencing depth increases [ Magoc et al. 2013]).
For all datasets, GeneStitch significantly improves the completeness of assembled genes as compared with initial assembly's genes (with higher gene coverage), and the number of complete genes, especially for the datasets with lower sequencing depths (6× or 13×).
The PBcR assemblies contained few collapsed repeats as compared with other assemblies (Supplementary Table S6), which is likely a product of longer, corrected reads.
As expected from the parameter optimization attempts (Fig. 4), this approach led to assemblies with less and larger contigs as compared with assemblies obtained under default settings (hss 4, bph 16) (Fig. 5, Additional file 6: Figure S4, Additional file 7: Figure S5).
Moreover, the lithographic SERS substrates can provide only a moderate enhancement as compared with some random assemblies [40].
MaSuRCA hybrid assemblies with PE-MP-454 combimprovedgeN50ated improvalues0 values but contained high number of contigs as compared with 454 only assemblies of four strains (Supplementary Table S3).
As a whole, the IIa/III/I region of BoLA is shifted ~3900 Kb telomeric in the Btau_4.0 assembly compared with the Btau_3.1 assembly.
