"In your genes we found 8,628 variations in the letters of your DNA as compared to the reference human genome.
However, as compared to the single-shrouded probe, the double-shrouded probe reduced the level of overestimation markedly.
There was no enrichment (eight probes, P = 1) within the top 500 ranked probes for known age DMPs, as compared to the remaining probes genome wide.
After converting the probe list from the 244K feature Agilent microarray to the latest genome build (NCBI 36.2), 15,684 regions were identified which, for at least one individual, lay between a probe previously scored as copy number variable and a probe which was unchanged compared to the reference sample i.e.e
All probes were compared to the reference sequence using BLAST (v.2.2.23).
The majority of these probes were hypomethylated in cerebellum as compared to the three cortical regions.
When investigating MYCN status by Fluorescence In Situ Hybridization (FISH), MYCN amplification is defined as >4-fold MYCN signals compared to 2p reference probe signals.
Miller et al (Miller et al, 2006), using the strategy applied here (e.g. Schwann cell referencing) but using chips with lower number of probe sets as compared to those studied here, identified 162 probe sets differently expressed in MPNST cell lines and MPNST tumours compared to normal Schwann cells.
Recently, an 8 F intracardiac probe was developed [ 28], which is a further improvement, as the intracardiac probe is much smaller compared to the current pediatric transesophageal probes.
As expected, the alternative probes (probe-pairs) displayed a generally lower ΔTm for shorter mutant probes or higher ΔTm for longer mutant probes when compared to the Tm-matched pairs.
