Total protein content was determined either with the Bio-Rad Protein Assay kit (Bio-Rad Laboratheies) or the BCA (bicinchoninic acid) Protein Assay Kit (Pierce) as appropriate, using BSA as a standard.
Statistical significance was evaluated by Fisher's exact test for group correlations, Mann-Whitney U test, Kruskal-Wallis test, student's t-test, logistic or linear regression analysis, as appropriate, using the SPSS software package (version 18, SPSS Inc, Chicago, Illinois).
Statistical analysis was made as appropriate using the XLSTAT software.
Statistical tests were adjusted for multiple comparisons as appropriate, using the Sheffe method.
Fisher's exact test was performed with two-tailed probability to detect the differences between groups as appropriate using Medcalc statistical software (Medcalc, Mariakerke, Belgium).
The statistical analysis performed using one-way analysis of variance (ANOVA) followed by Dunnett's post hoc test as appropriate using SPSS 19 software whereas open field and hole cross tests analyzed by ANOVA with repeated measures.
These conditions gave an analysis time of 17.33 min. The stock solution (1000 mg/L) for each ester was prepared in a 20 ml volumetric flask and diluted as appropriate, using a mixture of the phthalates and internal standard (I.S) at 1000 mg/L concentrations with ten replicate injections (1 μL).
Animals were genotyped as appropriate using DNA from either toe or tail snips using standard protocols.
Resulting contigs from both assembly methods were manually inspected for quality and joined as appropriate using an in-house sequence editor.
Results are expressed as mean±SEM and were analyzed by either Mann and Whitney test, one or two way ANOVA as appropriate, using PRISM 4.0 software.
Results are expressed as mean ± SD from 3 4 independent experiments and analyzed by means of unpaired Student's t-test, the Z-test for differences in proportion, or one-way ANOVA followed by Dunnett's post hoc test, as appropriate using GraphPad Prism 5.0 (GraphPad Software, Inc. La Jolla, CA, USA).
