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In each region, a reference node is considered and shown as an underlined alphabet, as shown in Fig. 8.
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The primers are as follows: 5'-TTGGATCC andGATATGATGGAGGAGGA-3' and 5'-TCACTCGAGGAACTTGTGCCACTGATGATCA-3' with an underlined BamH I site and Sal I site, respectively.
Often you will be given options on changing an underlined phrase to improve the mechanics or style, usually with a "no change" option as well.
Clicking on an underlined name takes the user to that person's Buzz profile page.
A new spelling checker lets you tap an underlined word to see correction suggestions.
a underlined: ribosomal binding site (modified KOZAK sequence).
Given these assumptions Table 1 shows all theoretically possible reactions to form pyocyanin whereby pyocyanin is regulated either via PqsE and therefore by PqsR (labeled as underlined) or via PqsA, PqsBCD, and therefore PqsR (as bold).
The primers used were as follows (the underlined: restriction sites).
In order to match the behavior of our computational model to the existing knowledge about pyocyanin formation (PqsR, PqsA, PqsBCD, and PqsE are required), we either have to combine a reaction labeled as bold with a reaction labeled as underlined or take an italic labeled reaction.
Factor V3K was constructed using the mutagenic primers 5′-C ATT TGG A A G T T T GCA CCT G-3′ (forward) and 5′-C AGG TGC A A A C T T CCA AAT G-3′ (reverse) (corresponding to DY → KF mutations), using factor VKFKF as a template (bold underlined letters identify the mutated bases) (19).
It had clearly been proven in the eating, but what are the ingredients which, particularly in the UK, simultaneously give the recipe-as-poem its credentials as comic verse, underlined by representation in such anthologies as The Nation's Favourite Comic Poems?
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com