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A 1% aliquot was preserved as an input sample and frozen at −80°C until the reverse crosslinking step.
The beads were pelleted by centrifugation and the supernatant containing the chromatin was aliquoted: a 100 µl aliquot as an input sample not immunopreciptated, and four aliquots with equal amount of the remaining supernatant (∼700 1000 µl each).
One tenth of the lysate was removed as an input sample.
Immediately after precleaning and before incubating with antibodies, a portion of the B73 reference sample was removed and set aside as an input sample.
Similar(56)
It contains a monoclonal and a polyclonal NRSF ChIP sample as well as a control input sample.
Part of the nucleic acid sample was set aside as an untreated input sample for comparative sequencing.
Immunoprecipitated methylated fragments, as well as input samples, were recovered using the IPure kit (Diagenode).
Parametric methods address the problem as a modeling of a stationary process, where the resulting representation allows to generate a new video with similar characteristics as the input sample [2 7].
A 50-μl sample was taken as the input sample, and the extracts were pre-treated with 0.6 mg of lysis-buffer-equilibrated Dynabeads Protein G (Invitrogen) (to avoid non-specific binding of DNA to the Protein G).
After sonication, the material was centrifuged at 16,100 × g for 10 min at 4°C and a 20 μl aliquot removed to serve as the "input sample".
An aliquot (5 μL) was saved as the input sample.
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