Sentence examples for as an established method from inspiring English sources

The density of immunostaining was measured using Image-Pro Plus Version 6.2 software (Media Cybernetics Inc., Bethesda, MD), described previously as an established method [ 4].

Recently, due to improvements in freezing of oocytes by vitrification, the American Society of Reproductive Medicine has also introduced vitrification of oocytes as an established method and the updated American Society of Clinical Oncology guidelines reflect this change.

The influence of the clinical data on the tissue response was tested for significance by performing an ANOVA with LSD-modification according to Bonferroni as an established method for comparative experiments in which only the difference in outcomes is of interest.

Conjugating biomolecules with polyethylene glycol (PEG), a process known as pegylation, is now an established method for increasing the circulating half-life of protein and liposomal pharmaceuticals.

Thomson (also referred to as Compton) scattering sources are an established method to produce appropriate photon beams.

The content of antibiotic entrapped in liposomes (after disruption with 0.2% Triton X-100) was measured by an established method as described previously for tobramycin and polymyxin B [51], [58].

It should be noted that clinical effectiveness of using the overall immune activation by application of bacterial cell components (i.e., BCG) into the urinary bladder tract for bladder cancer seems a more logical approach as a worldwide established method [79, 97, 98].

By using TUNEL (terminal deoxynucleotidyltransferase-mediated UTP end labeling) staining as a well established method for detecting DNA fragmentation, we could show that Gal-4 treatment robustly increased apoptosis of mucosal mononuclear cells (24.8±2.1%), whereas in control animals only few apoptotic cells were detected (5±1.8%) (Fig. 6B, C).

The primers and probes as well as the PCR conditions were the same as we used in a previously established method [ 17].

Apoptosis was assessed by a well established method as previously described [ 5, 9, 10, 15].

Moreover, the data can be used by Health Department/Ministry politicians, patient organizations, health insurance institutions, and pharmaceutical companies to evaluating drug safety over time, as a supplement to established methods such as spontaneous reporting systems.