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As an additional validation test, we created the [Cu25SR18]− NC (Fig. 2 iii)) based on the crystallographic structure of the corresponding [Au25SR18]− NC.
As an additional validation of the utility of wide-field three-photon excitation, functional excitation is demonstrated by performing three-photon optogenetic stimulation of cultured mouse hippocampal neurons expressing a channelrhodopsin; action potentials could reliably be excited without causing photodamage.
As an additional validation, the global compound set (389,533) was subjected to randomly partitioned fivefold cross validation.
Of the additional compounds, Ethyl-3-hydroxybutyrate, a strong activator for ab3A according to [3], was not tested in [18], making it suitable as an additional validation point.
As an additional validation we performed chromatin immune precipitation on the GRE upstream of the caveolin-1 gene in a human lung carcinoma cell line (Figures 6, 7).
The similarity in Km obtained for substrate 2 here with the values reported previously using a traditional radiolabeled substrate [29] served as an additional validation of the present fluorogenic red-shifted format.
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The model was applied successfully to predict PICC for different residual plastic fields which provided an additional validation of Δyp as the parameter controlling PICC.
This study provides an additional validation of rs300774 as a potential transdiagnostic biomarker for suicide attempts and evidence that ACP1 may have an important role in regulation of the multiple systems associated with suicide.
1) DIPLOMA VALIDATION PAGE: To ensure the Degree information is still valid, we highly recommend you visit the JHU official validation website to perform an additional validation.
However, a number of biological replicates or samples of the same condition as well as additional validation through qRT-PCR are necessary to eventually identify the biomarkers for class prediction on an independent validation set as the true changes in gene expression are often underestimated [ 32].
In addition, you might consider testing GFP LC3 II recruitment as an additional further validation in this assay.
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