Sentence examples for as an additional purification from inspiring English sources
As an additional purification step, CD4+ T cells were incubated with CD45RO-biotinylated antibody (Leinco Technologies, St . Louis MO; 0.1 µg/ml per 1×10∧6 cells).
In addition, an affinity column, for example, an Affi-Gel Blue Gel column, can be used as an additional purification procedure for the hSA fused protein.
GAG liberation and purification was conducted as described for guanidine-extracted PG with the exception of an additional purification step as follows to remove contaminating DNA in the tissue extracts: GAG-ethanol precipitates were solublized in 500 μl of dH2O, adjusted to a final concentration of 10% trichloroacetic acid, 0.01% BSA and incubated on ice for 1 hour.
It was concluded that this must be the product of the same gene with a His-tag extension, as this minor protein band could be removed by an additional purification step using a Ni-agarose column.
As mentioned above, the diheteroglycan partially co-elutes during the chromatography process with LTA and only an additional purification step following ion exchange chromatography yielded pure material in our study.
ChIP was accomplished using Dynabeads Protein A (Invitrogen) according to [ 44], except that following the Chelex-DNA purification, an additional purification with QIAquick PCR Purification Kit (Qiagen) was carried out; here, the ChIP-products were eluted in 50 μl and for subsequent qPCR measurements (as described above).
The ViroSeq system requires an additional purification step to remove dye terminators.
Initial affinity column purification was followed by an additional purification using a GE Superdex 200 2.6/60 on an Akta Purifier (GE Healthcare) in PBS containing 0.1% sodium azide.
DNA extractions were performed using a CTAB extraction method [ 74], with an additional purification using Qiagen mini-columns (Valencia CA).
All commercial reagents were synthesis grade and were used as received without additional purification.
All other commercial reagents were used as received without additional purification.
