Thus, in an additional experiment, we grew plants as described above but with N supplied as 3 mM NO3−+30 µM of labelled L-arginine.
The exact chemical nature of the discriminating feature has to be identified, a process that may involve searching for matches in existing databases as well as additional experiments using a battery of different analytical techniques.
Details regarding the synthesis and characterization of HcZ9, as well as additional experiments, methods, and a detailed protocol for the mobile zinc assay are available free of charge via the Internet at http://pubs.acs.org.org
Prioritized variants can then be followed up using Sanger sequencing, as well as additional experiments to categorize their phenotypic effect.
As an additional control experiment, we transfected U2OS-Δα-Nuc cells with luciferase reporter genes sensitive to cAMP (CREB), Ca2+ (NFAT) and glucocorticoid hormone receptor (GR) signaling.
Thus, as an additional control experiment, the authors should repeat the assay with a constant, small concentration (e.g. 1 μM) of monomeric and Lpd850-1250aa in the absence and presence of an excess (e.g. 5 μM) of actin.
Thus, as an additional control experiment, the authors should repeat the assay with a constant, small concentration (e.g. 1 μM) of monomeric and dimeric Lpd 850-1250aa in the absence and presence of an excess (e.g. 5 μM) of actin.
c As an additional control experiment we performed immunostainings for 8FM antibody and could demonstrate specific intranuclear inclusions in COS7 cells transfected with a construct expressing the FMRpolyG fused to GFP, while cells tranfected with only GFP did not show any inclusion formation.
This could potentially be extended to antibody-drug conjugates as well; additional experiments would be warranted to determine if the compartmental model were accurate for a particular antibody-drug conjugate (e.g., by comparing the cytotoxic drug residence to that of a surrogate radioisotope).
As a caveat, additional experiments were required to confirm the results from the RNA-seq data.
