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All strains were PCR verified, and mutations were additionally verified by DNA sequencing.
The attachment of the nanospheres was additionally verified by SEM imaging as shown in Figure 2a.
JNK activation was additionally verified by kinase assays as indicated by increased phosphorylation of JNK substrates in a time-dependent manner.
In 18 animals recording sites were additionally verified histologically using HRP or Flouro-Gold following the same procedures as in [22].
Behavioral states were additionally verified by video recording.
Recorded cells were additionally verified by post-hoc double-stainings for biocytin and respective reporter fluorophores.
Data on previous TB treatment was additionally verified among patients starting treatment through a medical record review.
Expression of the genes marked with have been additionally verified.
All the diagnoses were additionally verified using immunohistochemical examination.
Binding sites were additionally verified using the PISA algorithm [ 8].
As the overall perfusion level of the lower limb does not clearly distinguish between angiogenesis and arteriogenesis, the diminished growth of collateral arterioles was additionally verified by morphometrical analyses (Fig 5B and C).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com