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Plasma was used as a diluent to maintain a testing medium similar to that in the postchallenge LPS measurements.
Tap water as a test medium was essential to ensure sodium to calcium ion exchange in the bentonite layer.
We only tested one soil, but this fulfilled all the criteria in the test guidelines for natural soils used as a test medium.
Tocopherols as naturally present antioxidants influence considerably the antioxidant activity of other antioxidants added to plant oils used as a test medium.
Three different lipid matrices were used as a test medium for determining the antioxidant activity of phenolic acids and their alkyl esters (the test media were analyzed by peroxide value [ 20], p-anisidine value [ 21], fatty acid, and tocopherol composition; Table 1): Tocopherol-stripped sunflower oil was prepared according to the modified method of Waraho et al. [ 18].
The phosphate buffer (pH 6.8) [ 56] was maintained at 37°C and used as testing medium.
Plasma was used as a diluent to maintain similar testing medium, as in the post-challenge LPS measurements.
If plant oil is used as the test medium, there is a problem with the presence of naturally occurring antioxidants (especially tocopherols), which may have interactive effects on the antioxidant activity of the antioxidants studied.
A synthetic material made to simulate bicortical bone was used as the test medium.
Also, the background luminescence of the sensor strains (varying among the strains; Tables 1, 2, 3, 4) did not correlate with their sensitivities to the metals present as impurities in the test medium.
Mueller Hinton broth for bacteria and RPMI-1640 medium for yeast strain were used as the test medium.
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