Sentence examples for as a template for the second from inspiring English sources

30 ng of DNA was used in the first PCR, and 1 μl of 1 10 diluted amplicons from the first PCR were used as a template for the second run.

The reaction products from the two tubes were mixed and this mixture was used as a template for the second pre-amplification.

An aliquot (2 µl) of the PCR product was used as a template for the second PCR (35 cycles; annealing at 54°C, first five cycles: 2 min, remaining cycles: 45 s) using nested, subunit-specific primers (Table S1).

The mixture of the two PCR fragments served as a template for the second PCR reaction using primers 5' VPR BamHI and VPRVPstPst I only (Table 5).

One microlitre of the first PCR served as a template for the second nested PCR amplification, using gene-specific inner primers labelled at the 5'end with infra-red dyes IRD700 and IRD800 (see Table 4, LI-COR®, Lincoln, Nebraska, USA).

One µl of the first PCR served as a template for the second nested PCR amplification, using combination of specific primers carrying M13 tail and M13 universal primers, M13F700 (5'-CACGACGTTGTAAAACGAC-3') and M13R800 (5'-GGATAACATTTCACACAGG-3'), labelled at the 5'end with infra-red dyes IRD700 and IRD800 (LI-COR®, Lincoln, Nebraska, USA), respectively.

Urea and EDTA were selected as added fuels and sterile gauze was used as a template for the first time.

During embryogenesis, these bundles are thought to act as a template for the first cuticle layer.

Use the first panel as a template for the remaining three pieces (four in total).

Resulting first-strand cDNA was used as a template for the amplification of Fab fragments.

This product was used as a template for a second PCR using primers containing the full-length T7 promoter sequence.