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The resulting PCR products were serially used as a template for additional rounds (four total) of error-prone PCR, each time using 0.1 ng template per reaction.
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With the Beverly Hills boutique set to serve as a template for two additional U.S. stores that are scheduled to open before year's end — in Bal Harbour, Fla., and New York City — and the label on the cusp of its 20th birthday, does this mean the brand known for a witty blend of naughty and nice is toning down and growing up?
Found in plants, fungi and invertebrate animals, this is considered an amplification or enhancement step where RdRP synthesizes a complementary strand of the target transcript to form dsRNA that serves as a template for Dicer and additional small RNA production (Cogoni & Macino 1999; Dalmay et al. 2000; Smardon et al. 2000; Sijen et al. 2001).
The silica, intrinsically present in natural RH, was used as a template for mesopores, and BS was used for additional development of micropores.
One μL of liquid culture was used as a template for colony PCR (primer sequences in Additional file 7).
First-strand cDNA was synthesized from 1 μg of total mRNA, as described above, and applied as a template for qPCR with gene-specific primers (Additional file 4).
First-strand cDNA was synthesized from 2 μg of total RNA as described above and used as a template for real-time PCR with specific primers (Additional file 8, Table S8).
The resulting cDNA was used as a template for quantitative PCR with the primers listed in Additional file 2 using a Faststart Universal SYBR Green Master (Rox) kit (Roche).
Ongoing and additional studies are required to enable transcriptomic profiling to be useful as a diagnostic technique and as a template for treatment and biomarker discovery.
As a template for reform, this is a starting point.
Indian executives refer to China as a template for development.
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