Sentence examples for as a template for a secondary from inspiring English sources
The resulting PCR mixture is then used as a template for a secondary or "nested" PCR with the nested adaptor primer (AP2, provided by the manufacturer) and the nested gene-specific primer (GWRUT C30gsp2; Table 1).
For singleton validation, the entire gene was amplified directly from the whole genome amplified DNA using iProof high-fidelity DNA Polymerase (BioRad), and this full-length amplicon was then used as a template for a secondary PCR that used internally nested primers to robustly amplify the gene region containing the singleton to be validated.
RRF-1 amplifies the RNAi response by using the mRNA as a template for producing secondary (2°) siRNAs, which ultimately direct the cleavage of the targeted mRNA by the enzyme, CSR-1 [7].
PAA are being used mostly as a membrane [7], as a biosensor [8], and as a template for fabrication of secondary nano-meter scale materials [9].
Of this reaction, 1 μl was used as a template in a secondary PCR reaction performed under the same conditions, with the exception that primers were replaced by primers designed to overlap the compatible adaptors, barcodes and indexing for Illumina multiplexing sequencing35 for 20 cycles and a final extension at 72 °C for 5 min.
These results suggest that the second anodization is necessary to obtain open tube morphology with a uniform height throughout the entire sample without the bundling problem, which can be used as a template for easy deposition of secondary materials [37].
As a template for reform, this is a starting point.
Next, linear DNA template was prepared using P22 and P23 to be used as a template for in vitro transcription.
