Sentence examples for as a template for a second from inspiring English sources

Serial array capture was performed over two copies of each array using the enriched fraction from the first round of capture as a template for a second round.

Plasmids were isolated and used as a template for a second PCR with primers designed for cloning into the pEHISTEV vector (NcoI/HindIII), in order to add the His-TEV tag at the N-terminus of the protein.

The second proposed mechanism, known as semiconservative replication is when each strand of the parental DNA acts as a template for a second newly-synthesized complementary strand, which then combines with the original strand to form a DNA molecule.

In a nested PCR, the initial PCR reaction is performed on the extract and the product of this first reaction is then used as a template for a second PCR reaction.

A thousand-fold dilution of this PCR product was used as a template for a second PCR reaction, with the same forward primer and as a reverse primer 5'-CCATCGATTTCCAAAAAATACCAGAGTCTTACTAAATCTCTTGAATTTA-3' (the ClaI site indicated in italics and the Kaiso target sequence in bold).

This product was used as a template for a second PCR using primers containing the full-length T7 promoter sequence.

These products were used as template for a fourth PCR using overlapping primers to create gene specific amiRNA [ 51].

First-strand cDNA was synthesized by reverse transcription and was then used as a template for PCR amplification.

We used one tenth cDNA as a template for a PCR reaction using Amplitaq Gold polymerase (www.appliedbiosystems.com).

The first PCR product (1 : 100 dilution) served as a template for the second PCR.

The normalized cDNA was used as a template for the second run of LD-PCR.