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Birth size was included as a supplementary variable, because it contained information useful in estimating birth weight.
In order to further substantiate the relationship between lin system diversity and degradation efficiency, principle component analysis (PCA) was performed on the copy number and sequence divergence for these strains, with HCH degradation plotted as a supplementary variable to visualize correlation.
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Principle component analysis (PCA) with the copy number and divergence data was then done with the R package FactoMineR [ 75], with HCH degradation plotted as a supplementary discrete variable (0- complete non degrader, 1- partial degrader, 2- full degrader), followed by a plot construction with ggplot2.
For the partial Mantel test on MM + FF + MF dyads, we included sex as a supplementary co-variable (see Additional file 2).
Approximated models for these components and technique for approximation (such as supplementary variable method) should be investigated.
On the contrary, diatoms and filamentous cyanobacteria resulted in preferences of Bacilliariophyceae and Cyanophyceae (included as supplementary variable in the DCA analysis) to the Bofedal (B).
Patient-reported side effects, compliance and treatment satisfaction were considered as supplementary variable.
Non-contributory variables, as manifested by very close localizations of the categories of a variable on the graphs, were retained as supplementary variables in an attempt to help further interpretation [ 34].
The addition of sample annotations as categorical supplementary variables is used to attach a biological meaning to each component.
As such, supplementary variables significantly associated with the classes were used to describe patient profiles within each class.
Reasons for condemnation and portions with percentages lower than 1% were used as supplementary variables.
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