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Site-directed mutagenesis was performed by overlap extension PCR51,52 by using the recombinant plasmid pMCB17 containing a ClpB gene from Thermus thermophilus as a template (Supplementary Table 2).
Reverse transcription-polymerase chain reaction (RT-PCR) was performed with RNA from the DMV-infected plants as a template (Supplementary Fig. 1).
To confirm that LAVA is the repeat that expanded in the hoolock centromeres, we designed a forward primer in the AluSz6 portion (AluSF) and a reverse primer in the L1ME5 portion (L1MR) (fig. 3 B and supplementary table S1, Supplementary Material online) using as a template, one of the full-length elements identified in NLE.
Many people do this as a supplementary income.
Other schools are using Amazon as a supplementary course materials provider, Mr. MacDonald said.
David S. Rapkin provides sound effects ample enough to qualify as a supplementary electronic score.
Designate each item as a Supplementary File and number accordingly: for example, 'Supplementary File 1'.
Capacitor switching acts as a supplementary method.
A derivation of Eq. 1 from PET nomenclature is provided as supplementary information (Additional file 2), as is a template spreadsheet for calculation of ftb (Additional file 3).
The dominant (001) orientation is a result of the PFBT-treated Au acting as a templating structure (Supplementary Fig. 7), with the fluorine atoms of the PFBT molecules interacting with the periphery of diF-TES ADT molecules26.
In vitro transcribed fragments of 28 S rRNA were generated in a similar way using human genomic DNA isolated from placenta as a template for PCR reactions (supplementary table S1, Supplementary Material online).
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