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The BMD, derived from animal cancer data by mathematical modelling within the observed range of experimental data, is recommended as a standardized reference point.
Relative expression levels of genes of interest (GOI) were calculated as ΔCt values as follows: ΔCt = 2 0 − (Ct GOI − Ct (mean of RPL37A, CALM2, OAZ1 ) ). No-template controls as well as a standardized reference RNA (Stratagene qPCR Human Reference Total RNA, Agilent Technologies, Waldbronn, Germany) were used as controls.
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YD and DYD were then corrected for heterogeneity of variance and expressed as in a standardized (reference) environment.
As positive controls of RT-qPCR assays a standardized reference RNA (Stratagene qPCR Human Reference Total RNA, Agilent Technologies, Böblingen, Germany) and Human Genomic DNA (Roche Applied Bioscience, Mannheim, Germany) were tested on each plate.
Then, local microclimate data are used to determine a standardized reference evapotranspiration for the local microclimate (Em).
These anatomical landmarks allowed registering all morphologies into a standardized reference system (Fig. 1 B ).
Dataset 5 uses a reference sample design, where RNA from each experimental condition is co-hybridized on an array with a standardized reference RNA sample.
The occlusal surfaces of the crowns were then flattened to achieve a standardized reference point to determine the working length.
Despite introduction of a standardized reference measurement technique, widespread use of CDT remains limited due to low sensitivity.
Protein biomarkers have been used clinically as a standardized laboratory reference to diagnose and differentiate the status of both health and disease.
For assessment of basic creatinine level, the creatinine clearance formula for modification of diet in renal disease (MDRD) was used with 75 ml/minute*1.73 m as the standardized reference clearance [ 21].
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