Exact(1)
In the latter step, the normalization means that all dkiter m will be divided with a proper constant and overwrite as a standard normalization trick, that is, if the integral in Equation 8 via Equation 6 yields a value c, not necessarily N, then dkiter m:= dkiter msqrt(N/c).
Similar(59)
Murine Cyclophilin A RNA was used as a standard for normalization.
The transcription level of CaACT1 was used as a standard for normalization.
There are also publications on Ca using seawater as a standard for normalization in the bracketing method.
As the numbers of mapped reads in the two skip mutant samples (skip1 and skip2) and two wild-type samples (wt1 and wt2) are different (28465249 reads in skip1, 20849546 reads in skip2, 44865242 reads in wt1, and 18343654 reads in wt2), we take the minimum read number 18343654 as the standard normalization scale (SNS).
Then the total RNA was used forqRT-PCR analysis for specific mRNA with GAPDH as a standard housekeeping gene for normalization in using SYBR green mastermix [26].
Human serum albumin was used as a standard control for protein normalization.
The 36B4 gene was used as a normalization standard.
GAPDH mRNA was used as a normalization standard.
gapdh was used as a normalization standard with the primers listed in RT-PCR.
Since the expression of 36B4 varied among samples, GAPDH was also included as a normalization standard for cDNA input.
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