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HBIM is commonly intended as a specific library of parametric smart objects [21, 62].
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The expression level of an sRNA in a specific library was defined as the number of this sRNA divided by the total number (in millions) of genome-mapped sRNAs in this library, which was designated as 'RPM'RPM
An orthologous cDNA to dcx was identified as a library specific gene.
The ortholog for one of these factors (encoded by a gene called apterous (ap) in Drosophila) was identified as a library specific transcript (EZ421958).
qRT-PCR revealed wound stress protein (gi51457947), one of the abundant cDNAs in JNF library, as a specific cDNA to "off year" leaves in July.
The cDNA clones selected for full-length sequencing were from a non full-length enriched white muscle tissue specific library constructed as described in Adzhubei et al. [ 10].
First, in the probe screening method, CES were obtained by dot hybridization on membranes using DNA fragments derived from chromosome- specific library as probes to screen cDNA library or using cDNA fragments as the probe to screen chromosome-specific library.
50 100 ng of polyA RNA was used in a strand-specific library preparation as per the SOLiD Total RNA-Seq Kit protocol (Invitrogen, cat#4445374) and AMPure XP beads (Agencourt, cat A63881) were used for purification of cDNA and amplified DNA.
In order to get a general as well as a specific view, we used both the total 37,787 ESTs of the original library and the assembled distinct transcript library.
A series of overlapping clones were isolated by screening the library using full length cIRK1 cDNA as a specific probe.
In our previous study by a yeast two-hybrid screening [ 21], mEH was identified as a specific binding partner for HBSP from a human liver cDNA library.
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