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IFN-γ has often been used as a single readout for Th1 responses, but recent studies of other infectious diseases have emphasized the importance of polyfunctional T cells co-producing IL-2, IFN-γ and TNF-α [14], [47].
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Traditionally, the identification of gene products and neurons involved in behaviors such as thermosensation has relied on manual assays with a single readout - for example, a thermotaxis index measuring how well animals navigate toward the cultivation temperature.
Whereas a simple microarray experiment consists of a single perturbation and readouts for tens of thousands of genes, this screen includes thousands of cDNA overexpression perturbations and a single readout.
In contrast, most non-nuclear two-hybrid assays and PCAs rely on a single readout, thereby limiting throughput and increasing the incidence of false positives.
For our data, each weight is associated with a single readout of an independent siRNA.
HCS has already proven successful as a method to deliver more relevant information simultaneously in one experiment, rather than delivering a single readout in a series of sequential experiments [ 8– 11].
A criticism of this study would be its reliance on a single readout of Akt pathway activation.
Using SUMO foci dispersion as a single-cell readout of host SUMO proteome remodeling, we found that cells expressing actively replicating IAV RNP complexes had significantly fewer SUMO1 and SUMO2/3 foci than cells expressing all the viral protein components of the RNP in the absence of viral-like RNA or mock-transfected cells.
Ms. Quinn filed as a single taxpayer.
It treats you as a single person.
As a single parent, Godelieva was overwhelmed.
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