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The UV chromatogram shows elution as a single dimeric species.
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The compounds 1 & 2 contain a single dimeric unit while 3 & 4 contain two dimeric units.
Crossing over generates a single dimeric chromosomal structure, which is unable to segregate to the two daughter cells during cell division.
During the final chromatography step of purification, i.e. SEC, all three proteins were eluted as a single species with approximate mass of 40 kDa and native gels also identified that a single dimeric species was observed in each case.
Both monomeric and dimeric forms behave as a single species by ESI-MS under denaturing conditions and have a characteristic PYP UV visible spectrum, consistent with both species being holoproteins.
Although it is expected that Cif proteins function as monomers in vivo, each of the Cifs produced in this study displays the properties of monomer-dimer equilibria in solution, with the exception of CifBp which purifies as a single species (whose apparent molecular weight suggests a dimeric form).
By aligning the nucleosome signal (i.e., the smoothed positional distribution of mapped tag frequency; see Methods) to the central position of each type of Alu elements, we found one clear peak on the left side and another weaker peak on the right side in dimeric Alu elements as well as a single clear peak in both FLAMs and FRAMs.
This was confirmed by gel filtration analysis, which showed that the plant-derived mIL-10 eluted as a single peak with a profile corresponding to the size of the dimeric form.
The protein eluted as a single peak (Fig. 4B) with a retention volume corresponding to a dimeric assembly (∼114 kDa).
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