Sentence examples for as a second copy from inspiring English sources
Insertion of tagged PCNA as a second copy in this way did not cause slow growth in the absence of Elg1, nor was sensitivity to MMS significantly increased.
Note that our operational definition of a "single-copy" gene only requires that the fragment is not present as a second copy in the genome with similarity higher than 50%.
In contrast, if full-length DPB11 is expressed as a second copy in dpb11Δ C dot1Δ cells, the checkpoint defect is rescued to levels comparable to WT or dot1 strains.
To address this issue, we instead constructed strains with the POL30-3myc gene (under control of the native POL30 gene promoter) inserted as a second copy in addition to endogenous untagged POL30 gene.
For the strains in which the synthesis of ParA and ParB protein fusions was induced from the xylose-inducible promoter (pXyl) as a second copy, cells were grown in the presence of 0.03% xylose for 60 75 min (fluorescent ParB fusions) or 0.3% xylose for 1 hr (ParA-Dendra2).
To construct strains expressing POL30-3myc asecondcopy copy, a fragment spanning the tagged POL30 gene of TKY212 (524 bp upstream of POL30 to 3myc-kanMX cassette) was amplified by PCR and inserted into the his3 locus by homologous recombination.
As in pNDM-HK, a second copy of IS26 orientated in the same direction is often found near the mph2 gene at a distance.
After gaining momentum as a phylogenetic marker, a second copy of the gene, LWRh2, was subsequently discovered in bees, which fortunately appears to have evolved under a trajectory independent of the first copy, making both copies suitable for tree building [ 16].
A construct (TOG2-TOG2) in which TOG1 was replaced by a second copy of TOG2 rescued as well as did the wild-type (TOG1-TOG2).
The first integron (In-UMCU-1 accession no. AY987395), containing the qnrA1 gene, had the same additional structures as In36, orf513, qnrA1, ampR, plus a second copy of the 3´-conserved segment.
This is a trans-species aneuploid line expressing a large portion of Hsa21 (83%, 269 genes) as a third copy.
