The phrase "as a second assay" is correct and usable in written English.
It can be used in contexts where you are referring to a secondary test or analysis, particularly in scientific or technical discussions.
Example: "The results from the first assay were inconclusive, so we decided to conduct the experiment again as a second assay to verify our findings."
Alternatives: "as a secondary test" or "as a follow-up analysis".
As a second assay for the efficiency of autophagy induction, we tested the viability of yeast cells producing the long transcript when placed under autophagy-inducing conditions.
As a second assay, we also analysed TRIAD1 auto-ubiquitylation activity by immunoblotting (Supplementary Figure S1B), and found that this correlated well with UBCH7∼ubiquitin discharge data.
As a second assay, we measured the total number of viable cells in culture, irrespective of colony-forming potential, after irradiation at doses ranging from 0 to 5 Gy.
Cultures showing no amplification with this PCR assay were further identified by a second assay as described by Telenti et al and modified by Brunello et al [14] [15].
As a third assay to assess autophagic flux in our breast cancer cells, we took advantage of a Cherry-GFP-LC3B tandem construct.
As a first assay, we measured the activity of citrate synthase (CS), the enzyme controlling the access of acetyl coenzyme A in the tricarboxylic acid cycle, which is considered as an index of the mitochondrial mass in cells, as it is present exclusively in mitochondria.
We also developed a simple MLST protocol that could be used as a second confirmatory assay.
No diagnostic laboratory in Denmark uses WB as a second line assay to modify the specificity[ 26].
Conclusions: In view of the comparable results obtained with the two assays and of the ease of use, and higher throughput of HC-II, it seems advisable, with a single cytological specimen, to employ the HC-II test as a first-line assay, either for screening or diagnosis, and to perform reflex PCR on positive samples, if typing of prevalent high risk HPVs is needed.
The potential of higher plants as a first-tier assay system for detecting chemical mutagens is evaluated.
It is recommended that plant systems be accepted as a first-tier assay system for the detection of possible genetic damage by environmental chemicals.
