The method can be extended to include multivariate disturbances and, if a reference flux distribution as an operating point is available, to compute relative sensitivities.
Linear MoMA finds a solution that satisfies the same constraints as FBA while minimizing the Manhattan distance from a reference flux distribution.
More precisely, flux balance analysis predicts ratios of metabolic fluxes, i.e., values of metabolic fluxes relative to a reference flux that needs to be determined independently, for example through experimental measurements.
For each analysis, as the primary nutrient for yeast switched from A to B, we used the FBA predicted flux pattern for yeast as it grows on nutrient A as the reference flux.
We fix some experimentally determined exchange fluxes, and use geometric FBA [ 57] to choose a unique reference flux distribution consistent with the experimental data.
A unique reference flux (see additional file 2) is chosen from the space of all solutions to the above problem, by finding the box that defines the maximum and minimum values attainable by each v j, then choosing a flux as close as possible to the centre of the box.
The results suggest that this method could be used as a reference surface soil heat flux measurement.
The indirect system is based on a CCD camera that uses a water-cooled heat flux sensor as a reference for converting gray-scale levels into heat flux values.
The quantity (alpha c^* overline {v^* v^*}) on the last line of (24c) may be interpreted as a reference for the magnitude of the energy flux of mid-latitude RWs.
The non-null fluxes, instead, are subjected to a progressive reduction of 1% of the reference flux values for each simulation run.
