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Another membrane protein, Mp2CL5, was isolated from pathogenic N. fowleri and was not found in non-pathogenic Naegleria species, suggesting Mp2CL5 as a potential pathogenicity factor [ 19].
In the present study, we also identified vesicle trafficking as a potential pathogenicity mechanism (see the following section).
VPaI-7 is an 81 kb region that encoded a TTS system and the tdh gene and was previously identified as a potential pathogenicity island (Table 1) [ 19].
Toxb is a host specific chlorosis toxin and was identified as a potential pathogenicity factor in P. tritici-repentis, the causal agent of tan spot of wheat [ 26].
Formins, such as formin D, which we identified as a potential pathogenicity factor in N. fowleri (Table 4), are a crucial class of proteins that regulate the formation of actin filaments.
Because actin is localized around food cups and has been shown to have the capacity to modulate in vitro cytotoxicity in different target cells, it is frequently discussed as a potential pathogenicity factor in N. fowleri [ 11– 18] 18].
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Hence, xopF1 is a potential pathogenicity determinant in tomato.
We identified myosin II heavy chain as well as myosin Ie as potential pathogenicity factors in N. fowleri (Table 4).
Alternatively, molecular phenotypes, such as perturbation of chromatin structure or transcription factor binding, can serve as an indicator of potential pathogenicity and have been adopted by CATO and DeepSEA.
In F. oxysporum, four different Pex genes (PEX1, PEX10, PEX12 and PEX26) were identified as potential pathogenicity genes in a recent insertional mutagenesis screen, and the requirement for full pathogenicity was verified for two of them (PEX12 and PEX26) by complementation with the intact genes [ 59].
Further research should be conducted on LCMV in Spain to isolate autochthonous strains and establish their serologic and genomic characterization as well as their potential pathogenicity for humans.
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