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Assays (in triplicate) were repeated at least twice with the constitutive elongation factor 1α (ef1as as a normalizing control.
The GAPDH gene was used as a normalizing control.
100 ng of cDNA was used for the 25 µl PCR reaction, and GAPDH was used as a normalizing control.
Comparative quantitative analysis was performed using β-actin as a normalizing control and expressed relative to BMP receptor mRNA levels in dsRedΔWEHI cells.
GAPDH was used as a normalizing control.
(A ) Gapdh probe was used as a normalizing control.
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Assays were performed on the above noted gene products using human GAPDH as an endogenous normalizing control.
All reactions were always run in triplicate using 5S as normalizing control, as recommended by the manufacturer.
We used untreated cells and cells treated with RNAi directed at LacZ as parallel control, and measurement of Rho1 as internal normalizing control.
All miRNA primers were purchased from Qiagen and the relative expressions were calculated using the comparative CT method with mouse U6 small nuclear RNA as the normalizing control.
Beta-actin was used as normalizing control.
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