The phrase "as a normalization reference" is correct and usable in written English.
It can be used in contexts where you are discussing a standard or baseline used for comparison or adjustment in data analysis or scientific research.
Example: "We will use the control group as a normalization reference to ensure the accuracy of our experimental results."
Alternatives: "as a standard reference" or "as a baseline reference".
The histone H2B gene was used as a normalization reference (internal control) for target gene expression ratios.
Specifically, we identified a set of 11 detectable miRNAs on the TLDA card to serve as a normalization reference.
Other forms of stable functional activity, such as sleep-like slow wave activity, may also potentially be used as a normalization reference.
The actin gene was used as a normalization reference for target gene expression level, and wild-type before sorbitol treatment was set as the calibrator in each experiment.
Muscle is used as a normalization reference to determine intensity for a "permeable tissue" with no BBB, and the blood vessel region used to exclude pixels which represent vessels.
MiRNA expression levels were quantified by the ▵▵CT method (36) using the miR-183, co-expressed by the same construct, as internal normalization reference.
MiRNAs expression levels were quantified by the ▵▵CT method using either the U6 RNA (human) or the snoRNA 142 (mouse) as internal normalization reference.
The resulting fingerprints were compared using the Bionumerics version 6.5 software package (Applied Maths, Sint-Martens-Latem, Belgium) using the EZ load 100 bp PCR Molecular Ruler (Biorad) as normalization reference.
HsRNU6 was used as normalization reference gene for miRNA.
RNA polymerase B11a (RPB11Additionalonal file 1, Table S4) was used as a house-keeping gene for normalization (reference gene).
The use of reference RNA as a normalization tool in microarray experiments reduces bias due to dye incorporation, eliminate the need for dye swap, and can be used for multiple comparisons as detailed elsewhere [25].
