Sentence examples for as a normalisation control from inspiring English sources

U6 was used as a normalisation control for all tumour and adjacent normal lung tissues and cell line samples.

A constant amount of recombinant Tau was included in an individual lane on each Tau protein gel to act as a normalisation control.

The array plate was pre-coated with the RT-PCR primers of the 95 miRNAs and the U6 transcript was included as a normalisation control.

Spiked-in cel-miR-39 was analysed as a normalisation control and relative expression was calculated using the 2−ΔΔCt method (Livak and Schmittgen, 2001).

Equal amounts of protein were used (10 20  μg of total protein) and equivalent loading was assessed using β-actin as a normalisation control.

An identical amount of Renilla expression vector PRL-TK was co-transfected into every group to serve as a normalisation control.

As a suitable normalisation control has not yet been established for use in human serum, the use of absolute quantitation is at present the best way of comparing miRNA levels between individual patients/controls.

β- Actin served as an endogenous normalisation control.

β-Actin was used as the normalisation control.

The reactions were performed in triplicate and β-actin was used as endogenous normalisation control.

Cells were co-transfected with the NF κB-Luc construct and a Renilla luciferase reporter gene (Promega, Madison, WI, USA), which served as normalisation control, using Lipofectamine Plus (Invitrogen, Carlsbad, CA, USA) in F-10 medium, as described previously (Zbytek et al, 2008).