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Anglesey MP Albert Owen described it as a "negative step".
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He said it was a negative step while both sides were trying to send positive signals.
As a negative control, the step using the TUNEL reaction mixture was omitted, and a nucleotide mixture in reaction buffer was used instead.
Since the response to asialofetuin served as a negative control, a wash step was not warranted for routine experiments.
Yet the discussion has raged all season about whether City represent a positive or negative step for the W-League.
As a negative control, the secondary antibody step or the Thioflavin-T step was omitted, and no optical crosstalk was detected (data not shown).
We show that p66Shc acts as a negative regulator of all steps of the chemotactic responses triggered by CXCR4 and CXCR5 by inhibiting Vav-dependent actin cytoskeleton reorganization and provide insight into the mechanism by which p66Shc uncouples these receptors from Vav activation.
As a negative control, the primary antibody step was omitted, no staining was observed (data not shown).
A "No-Ab" sample was included as a negative control for the immunoprecipitation step.
To avert the risk of contaminating the DNA, we performed PCR amplification by using total RNA in the absence of the reverse transcription step as a negative control.
A BRSV positive cell culture was used for positive control while nuclease-free water (Amresco, Solon, OH, USA) was used as a negative control of the purification step.
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