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Cells from the same population that were not exposed to BrdU were used as a negative staining control to determine background-staining levels for the anti-BrdU monoclonal antibody.
Uranyl acetate was used as a negative staining agent.
As a negative staining control, we incubated CHO-K1 cells with both the ligand and receptor availability assay reagents to establish background staining levels.
As a negative staining control, normal goat IgG (Jackson Immunoresearch, West Grove, PA) diluted to 2 mg/mL in 4% BSA-PBS was used instead of the primary antibody.
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Absence of the BRAFV600E mutation is seen as a negative stain.
(Right) time-lapse series of EGFP-ezrin (green) and rhodamine-dextran (red) used as a negative stain in the culture media to detect the position of the cell membrane.
(C ) Time-lapse series of Emerald-mEps8 (green) and rhodamine-dextran (red) used as a negative stain in the culture media to detect the position of the cell boundary.
The staining intensities were scored as: - = Negative staining; + = Weak staining intensity; ++ = Medium staining intensity; +++ = Strong staining intensity.
As a negative control, staining was performed with mouse pre-immune serum instead of specific antibodies.
As a negative control, staining with HLA-A*0201 HLA-A*0201onegativentamer (ProImmune) was used.
As a negative control, staining was performed on selected sections with isotype control.
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