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The development of new diagnostic weights has been proposed as a method of adapting the general model – the Acute Physiology and Chronic Health Evaluation (APACHE) II in this case – to a new group of patients.
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A method of calculating vapour fraction is adapted to the calculation of rates of multicomponent condensation.
Total NAC (i.e. oxidised + reduced) was measured in deproteinised plasma using HPLC with fluorescence detection (as above) according to a method adapted from that of Wilkinson et al [ 28].
In addition, urinary As metabolites were speciated using a method adapted after Heitkemper et al. (2001).
Intracellular levels of pteridines were quantified by HPLC analysis using a method adapted from Fukushima and Nixon [20] as described previously [21].
The total number of km.tons was modelled using a method adapted from Dornburg and Faaij [ 34].
HPLC electrochemical detection of plasma-free choline was performed according to a method adapted from one previously described by Fossati et al. [ 23] and as reported previously [ 24].
We used a method adapted from [48].
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