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Another potential benefit of gut passage arises as a result of the removal of fruit pulp which otherwise may act as a germination inhibitor.
One hundred MG-20 seeds were soaked in sterile water as a germination control.
Intriguingly, the impact of MAX2 on plant auxin status is mediated via the proposed perception of a newly discovered phytohormone, strigolactone [ 31- 34], first identified as a germination stimulant for parasitic plants of the genera Orobanche and Striga (hence the name strigolactone) [ 35, 36].
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Conversely, rga28 and gai-t6rga28 14 DAR seeds still retained a significantly higher level of dormancy, as revealed by a germination rate of 30% and 22%, respectively.
The seeds could be germinated in vitro readily in a germination medium as reported previously (Huang et al. 2014).
Furthermore, some biological attributes of F. hodginsii seeds, such as a low germination rate (30 360 days), and a relatively hard seed coat (Nguyen et al. 2015), may have also contributed to the poor regeneration.
A germination test was conducted as for the wintering test with four replicates.
The physiological indices of seed germination were determined as follow: germination vigor (GV) = A/C, germination rate (GR) = B/C, germination index (GI) = Σ(Gt/Dt), and vigor index (VI) = GI × S. A is the total number of seeds germinated in 4 d, B is the total number of seeds germinated in 6 d, and C is the total seeds in the experiment.
Several C6 fatty esters of disaccharides have that composition, among them sucrose caproate, which has been reported as a seed-germination inhibitor [ 33].
The WGE comprises all components for translation, stored in a dried state, and ready for protein synthesis as soon as germination starts.
A QTL for this transgenerational correlation was mapped to a genomic region harboring one copy of FLOWERING LOCUS C, a genetic locus known to affect both days to flowering as well as germination phenotypes.
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