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The far-field set up has been discussed under the methods section (see Fig. 3). Figure 6(a) shows a typical reflectance spectrum as a function of the prism incident angle θ.
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The prism duct output beam dimension is determined as a function of distance from the prism exit face and the optimum condition for the maximum power transmission and best image quality is reported.
We note that the horizontal anomaly pattern does not change significantly as a function of prism height, only the bulk magnetization value of each prism does.
e Error estimates as a function of scattering angle for the PRISM simulations in b d.
The Kaplan Meier method (Graphpad Prism) was used to estimate survival probability as a function of time, and the log-rank test to examine differences in survival between subgroups.
The amount of FAM or VIC fluorescence in each reaction liberated by the exonuclease degradation of the TaqMan probe during PCR amplification was measured as a function of PCR cycle using ABI 7000 Prism (Applied Biosystems, Foster City, CA).
Raw data were normalized to baseline fluorescence (0%) and 5HT at 10 μM (100%), expressed as percent activation, and plotted as a function of molar concentration of test compound using Prism 5.0 (GraphPad Software).
Individual denaturation curves were analyzed and best-fitted by plotting the fraction of PrPSc remaining in the pellet as a function of GdnHCl concentration, and using a four parameter logistic equation (GraphPad Prism).
The Michaelis constant (KM) was determined by measuring and plotting AChE activity as a function of ATChI concentration and non-linear regression analysis (Prism software, GraphPad).
The product formation rates (as a function of dNTP concentration) were plotted to estimate the kinetic parameters Km and kcat by nonlinear regression fitting to the Michaelis Menten equation using Graph Pad Prism 5.0 software (GraphPad, San Diego, CA).
Corrected absorbance data were analyzed as a function of serum dilution by non-linear regression analysis using Prism software (GraphPad Software , Inc.
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