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Collecting these misincorporation frequencies into matrix T, we compute the PCR mutation frequencies P as a function of the polymerase misincorporation frequencies T resulting in the secondary null hypothesis (2) H ′ 0 : P = P (T ).
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The variable results obtained with Taq polymerase and the KAPA3G Plant PCR Kit indicate that PCR success and sequence quality may be as much a function of the taxon as the methodologies used.
RNA polymerase and ribosome concentrations were expressed as a function of the specific growth rate, μ, corresponding to the changes in the growth rate during batch cultivation.
DOI: http://dx.doi.org/10.7554/eLife.03164.009 Analyses of the distribution of RNA polymerase II (RNAPII) along the HSP70 gene as a function of the presence of eEF1A1 supported this conclusion, arguing that eEF1A1 is an activator of transcription upon stress.
In addition, on the basis of results for polymerases from different families, including pol β, the rate of single-nucleotide deletions increases as a function of the polymeric run length.
In this study the yield is analyzed as a function of several processes: (1) thermal damage of the template and polymerase occurring during the denaturing step, (2) competition existing between primers and templates to either anneal or form dsDNA, (3) polymerase binding to annealed products (primer/ssDNA) to form ternary complexes and (4) extension of ternary complexes.
A detailed description of the model underlying H ′ 0 and the computational challenges associated with computing the posterior distribution of P as a function of T are discussed under the 'Methods' subsection 'Modelling PCR via Polymerase'.
Analysis of the running start assays was performed as previously described by calculating the relative lesion bypass efficiencies (8-oxodG bypass%) of each polymerase as a function of reaction time.
9) The function of the "polymerase" domain of Cas10 is certainly open to question.
Gene expression of cells was determined with reverse transcription polymerase chain reaction (RT-PCR) as a function of hydrogel stiffness.
Our results corroborate a previous study of T7 DNA polymerase error rates within mononucleotide TRs as a function of repeat number (Kroutil et al. 1996).
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