Appendix 1 lists candidate tools, and we have classified them in three categories as a function of their determination mode, based on a now classical review (Kant 1996): a) indices based on intakes of nutrients (or at least of certain nutrients); b) indices based on the consumption of foods or of groups of foods; and c) indices that combine both approaches resulting in dietary patterns.
Reductive precipitation of U VI), resulting in removal of insoluble amorphous UO2 from solution, was measured as a function of time by determination of total dissolved U. Reductive precipitation was measured, rather than net U VI) reduction to U IV), to assess overall U removal rates from solution, which may be used to gauge the influence of chelation on microbial U mineralization.
The validity of the models was evaluated as a function of their respective coefficients of determination (R).
Measurements of dissolution rates as a function of temperature allow a determination of an apparent activation energy (Ea,app) for the process, via mass-loss calculations from image analysis.
Acquiring a series of spectra as a function of gradient amplitude permits the determination of diffusion coefficients for components that cannot be resolved in the one-dimensional (1D) 1H NMR spectrum.
For each substrate concentration, plots of velocity as a function of inhibitor concentration allow the determination of an apparent inhibition constant (KIapp).
The plotting of kcat and KM or their combination as a function of inhibitor concentration allows the determination of both the type of inhibition and the Ki value.
Following the determination of overall methyltransferase biochemical activity as a function of modulation of AUF1, we determined its effect on genomic DNA methylation (Fig. 3C).
Open image in new window Figure 1 Calibration curves for concentration determination as a function of optical density (a) 5-FU, (b) microspheres.
The plot of KIapp as a function of the substrate concentration allows the determination of KI when the substrate concentration is zero.
A replot of the initial rate of NRI-N-P dephosphorylation as a function of PII concentration (that is, a determination of the PII Kact), at low and high concentrations of α-ketoglutarate, is shown in Figure 2.
