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The initial rate of 2-hydroxymuconic semialdehyde was recorded as a function of absorbance at 410 nm over time.
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It represents the variation in absorbance as a function of current absorbance.
Change in absorbance at 688 nm was followed as a function of time at 25°C.
Absorbance was measured at the maximum wavelength as a function of radius at 25,000 rpm.
Absorbance was monitored as a function of the temperature at 295, 240, 260 nm for the determination of the melting temperature (Tm) [ 44] and at 405 nm as control wavelength.
(a) Absorbance and (b) photoluminescence spectra of CdS nanoparticles as a function of annealing time at 100°C.
Fig. 4 Variation of absorbance as a function of time of equilibration at 355 and 550 nm.
The time required for sorption of I3− into the sensor was studied by recording the absorbance of the I3− sorbed sensor at 355 nm as a function of time of equilibration at intervals of one minute.
A standard curve of absorbance at 660 nm as a function of protein concentration of the standard solutions was plotted and used to determine protein concentrations of the sample.
Extended Data Fig. 3 shows both the raw diffuse reflectance as a function of λ and the analogous absorbance calculated using the Kubelka-Munk23 function plotted against energy.
Increase in absorbance at 575?nm was recorded as a function of time against the reagent blank at room temperature (spectra 1).
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