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Carbamidomethylation of cysteine was specified in Mascot and X!Tandem as a fixed modification.
The iodoacetamide derivative of cysteine was specified in Sequest and X!Tandem as a fixed modification.
Iodoacetamide derivative of cysteine was specified in Mascot and Sequest as a fixed modification.
Iodoacetamide derivative of cysteine was specified in both search engines as a fixed modification.
Iodoacetamide derivative of cysteine was specified in Mascot as a fixed modification.
Carbamidomethylcysteine was selected as a fixed modification and oxidation of methionine as a variable modification.
Oxidized methionines were searched as a variable modification and alkylated cysteines were searched as a fixed modification.
The Paragon algorithm searched iTRAQ 4-plex samples as variable modifications with methyl methanethiosulfonate as a fixed modification [20].
The proteolytic cleavage was set to trypsin and the program was configured to report methylmethanethiosulphate (MMTS) as a fixed modification.
Searches were restricted to human taxonomy allowing carbamidomethyl cysteine as a fixed modification and oxidized methionine as potential variable modification.
Searches were performed using cysteine alkylation modification with the light form of acrylamide as a fixed modification and the heavy form of acrylamide (+3.01884) as a variable modification.
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