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As a first classification model verification strategy, we tested the model derived from the microarray data on the multiplex PCR data (11 samples).
As a first classification, we used MLST to determine the sequence type (ST for all prototype strains of each subgroup and cluster by sequencing internal fragments of 7 housekeeping genes (adk, fumC, gyrB, icd, mdh, purA, and recA) (26 ).
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Two separation factors, calculated from the retention of carotenoid pigments used as probe, allowed to draw a first classification diagram.
The use of meta-classifiers is a more sophisticated method of classifier combination, which uses the individual classifiers' outputs as input for a second classification step.
If precise data are not available (i.e., cytogenetic or molecular biology), a third classification of intermediate risk, as noted previously [ 5], may be needed.
Shupe wanted another option, a third classification, and found an ally in Portland lawyer Lake Perriguey.
Low and medium contact levels (the reference category)–As in the first classification (see above).
As a first, rough classification, the amino acid sequence of all predicted CDS were compared against the eggNOG database (evolutionary genealogy of genes: Non-supervised Orthologous Groups [ 20]; Table 2), and the results were compared to those obtained for the other completely sequenced Pseudonocardiaceae (Additional file 1).
As a "second generation" classification, ICF is concept driven.
In this proposal, as a first part of the classification process, the data was pre-processed to eliminate outliers and reduce the confusion.
These 30 cases were classified again by all 8 raters as part of the first classification session with the total 150 fractures.
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